Conversion of linear histogram flow cytometry data to a logarithmic display
نویسندگان
چکیده
منابع مشابه
Publishing flow cytometry data.
Cellular measurements by flow cytometric analysis constitute an important step toward understanding individual attributes within a population of cells. Assessing individual cells within a population by protein expression using fluorescently labeled antibodies and other fluorescent probes can identify cellular patterns. The technology for accurately identifying subtle changes in protein expressi...
متن کاملUsing flowViz to visualize flow cytometry data
UNLABELLED Automated analysis of flow cytometry (FCM) data is essential for it to become successful as a high throughput technology. We believe that the principles of Trellis graphics can be adapted to provide useful visualizations that can aid such automation. In this article, we describe the R/Bioconductor package flowViz that implements such visualizations. AVAILABILITY flowViz is availabl...
متن کاملLimits of Flow-Cytometry Histogram Analysis Methods to Assess Bladder Tumour Antigen Expression
Tumour-associated antigens detected in cells obtained from bladder washings or tumours are useful markers in bladder cancer. Flow cytometry is commonly used to quantify immuno-stained cells. A straightforward way to analyze data is to count the fluorescent cells above a threshold empirically determined on a control histogram representation. However, specific antigens expressed at highly variabl...
متن کاملFlow cytometry data analysis using Bioconductor - A typical work flow
The advent of high throughput techniques has resulted in the generation of large flow cytometry (FCM) data sets. Along with the task of acquiring the data comes the task of storing, managing, quality control, data analysis and data summarization to a condensed form that can be interpreted by the researcher. Open source Bioconductor packages for analysis of flow cytometry data provides a unified...
متن کاملStatistical file matching of flow cytometry data
Flow cytometry is a technology that rapidly measures antigen-based markers associated to cells in a cell population. Although analysis of flow cytometry data has traditionally considered one or two markers at a time, there has been increasing interest in multidimensional analysis. However, flow cytometers are limited in the number of markers they can jointly observe, which is typically a fracti...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Cytometry
سال: 1982
ISSN: 0196-4763,1097-0320
DOI: 10.1002/cyto.990030213